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目的:比较荧光原位杂交(fluorescence insitu hybridization,FISH)和免疫组织化学在检测间变性大细胞淋巴瘤(anaplasticlargecelllymphoma,ALCL)中间变性淋巴瘤激酶(anaplasticlymphomakinase,ALK)基因转位及其融合蛋白中的作用。方法:采用双色FISH和免疫组织化学检测30例石蜡包埋ALCL病例中ALK基因转位及其融合蛋白。结果:FISH和免疫组织化学分别在60.7%(17/28)、71.4%(20/28)系统性ALCL中检测到ALK基因转位或融合蛋白,在2例皮肤原发ALCL中均未检测到基因转位或融合蛋白。结论:在检测ALCL中有无ALK基因转位时,ALK蛋白免疫组化由于其简单、快捷、价廉成为一般情况下的首选方法;在具备FISH条件时,由于其准确性也可以将检测ALK基因转位作为首选。
OBJECTIVE: To compare fluorescence in situ hybridization (FISH) and immunohistochemistry in the detection of anaplastic lymphocyte kinase (ALK) translocation and its fusion protein in anaplastic large cell lymphoma (ALCL) effect. Methods: ALK gene translocation and fusion protein in 30 ALCL cases were detected by two-color FISH and immunohistochemistry. Results: ALK gene translocation or fusion protein was detected in 60.7% (17/28) and 71.4% (20/28) systemic ALCLs respectively by FISH and immunohistochemistry, but not in both skin primary ALCLs Gene translocation or fusion protein. Conclusion: In the detection of ALK gene translocation in ALCL, immunohistochemistry of ALK protein is the preferred method in general because of its simplicity, quickness and low cost. In the case of FISH, its accuracy can also be used to detect ALK Translocation as the first choice.