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目的:探讨新型低温细胞生存系统用于太空无源搭载的可行性。方法:小鼠黑色素瘤B16细胞在低温细胞生存系统培养26天,利用光镜、MTT法、FCM观察细胞的生理特性;荷瘤小鼠观察致瘤性及对免疫系统的影响。结果:新型低温系统使细胞生理特性有一些改变,此变化是可逆的;对细胞的形态、致瘤性及免疫系统没有显著影响。结论:此系统是目前最简单、易行的无源搭载装置,适合于研究空间生物学效应。
Objective: To explore the feasibility of using new cryogenic cell survival system for space passive loading. Methods: The mouse melanoma B16 cells were cultured in hypothermia cell survival system for 26 days. The cell physiological characteristics were observed by light microscopy, MTT and FCM. The tumorigenicity and immune system were observed in tumor-bearing mice. RESULTS: The new hypothermia system changed the physiological characteristics of the cells. The change was reversible. There was no significant effect on cell morphology, tumorigenicity and immune system. Conclusion: This system is by far the simplest and easiest passive device to fit the space biology.