The effects of ozone ice on the shelf life of Pseudosciaena crocea and cuttlefish, stored under refrigeration (4±0.5℃) were studied by monitoring the microbiological, chemical and sensory changes for a period of 17 days. Results showed that the shelf life could be lengthened 4～5 days preserved by 4mg/kg ozone ice. The total volatility basis nitrogen (TVB-N) was reduced and the bacteria colony was decreased by 92%. The bactericidal effectiveness of ozone ice is strong enough to attain better preservation effect. As a convenient, simple and environmental friendly method, it could be used in the preservation and transportation of fishery products.