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目的研究曲马多在中毒家兔体内死后分布规律,为曲马多中毒检材采取提供实验依据。方法家兔经口给予10倍LD50曲马多,待家兔死亡后迅速解剖取样,气相色谱/质谱联用和气相色谱-FTD法测定其体液、脏器、大脑及右上肢和右下肢肌肉中曲马多的含量,比较其变化规律。结果血液和肝脏中曲马多的最低检出限分别为0.05μg/mL和0.05μg/g,提取回收率为97.60%±0.65%~103.10%±1.24%。曲马多在家兔体内的死后分布为:肾>胃>肝>脾>肺>脑>心>上肢肌肉>下肢肌肉>>体液(尿>胆汁、心血>玻璃体液)。结论大剂量曲马多中毒致死后在体内分布不均匀,组织中曲马多含量明显高于心血、胆汁等体液。
Objective To study the distribution of tramadol in rabbits after poisoning, and to provide experimental evidence for the detection of tramadol poisoning. Methods Rabbits were orally given 10-fold LD50 of tramadol. The rabbits were dissected quickly after death and their body fluids, organs, brain and right upper extremity and right lower extremity muscles were determined by gas chromatography-mass spectrometry and gas chromatography-FTD Tramadol content, compare the changes in the law. Results The detection limits of tramadol in blood and liver were 0.05μg / mL and 0.05μg / g, respectively. The recovery rates of tramadol were 97.60% ± 0.65% ~ 103.10% ± 1.24%. Tramadol in rabbits after the death of the distribution as: kidney> stomach> liver> spleen> lung> brain> heart> upper extremity muscle> lower extremity muscle >> body fluid (urine> bile, blood> vitreous humor). Conclusion The high dose of tramadol poisoning caused by in vivo distribution is not uniform, the content of tramadol in the tissue is significantly higher than that of blood, bile and other fluids.