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经菜粉蝶幼虫增殖的大菜粉蝶颗粒体病毒(PbGV),其包涵体为卵圆至椭圆形颗粒,不易为电子束所透射;病毒粒子略呈杆状,稍弯曲,脂膜内外有电子致密物质沉积;核衣壳杆状,较直。病毒包涵体、病毒粒子和核衣壳的大小分别为335-398×219-259nm、221-260×65-75nm和245-283×51-62nm。 PbGV包涵体经口喂饲菜粉蝶初孵幼虫,其致死中浓度(LC_(50))为1.22×10~3GIB/ml。幼虫饲毒后,致死中时(LT_(50))随病毒浓度提高而缩短;随饲毒时虫龄的增大而延长。病毒感染寄主幼虫的最适温度约为25～30℃,在此温度范围内,LT_(50)明显缩短。
The cabbage butterfly larvae proliferation of cabbage butterfly virus (PbGV), its inclusion body is oval to oval-shaped particles, not easily transmitted electron beam; virus particles slightly rod-shaped, slightly curved, inside and outside the lipid membrane with electron-dense material Sedimentation; nucleocapsid rod-shaped, straight. The size of virus inclusion bodies, virus particles and nucleocapsids were 335-398 × 219-259 nm, 221-260 × 65-75 nm and 245-283 × 51-62 nm, respectively. PbGV inclusion bodies were orally fed with newly hatched larvae of B. bassiana, and their lethal concentration (LC 50) was 1.22 × 10 ~ 3GIB / ml. When the larvae were lethal, their lethality (LT 50) decreased with the increase of the virus concentration and prolonged with the increase of the instar larvae. The optimum temperature for virus infecting host larvae was about 25-30 ℃, and LT 50 was significantly shortened in this temperature range.