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以麻黄幼苗为试材,利用PEG-6000人工模拟干旱胁迫,采用盆栽灌根法,研究了腐殖酸、外源硝普钠(SNP)、甜菜碱处理下麻黄幼苗的可溶性蛋白质、丙二醛(MDA)含量及抗氧化酶活性的变化。结果表明:干旱胁迫下,麻黄幼苗MDA含量显著升高,可溶性蛋白质含量及过氧化物酶(POD)、过氧化氢酶(CAT)活性降低,超氧化物歧化酶(SOD)活性升高;对比干旱胁迫,外源物质处理下MDA含量明显降低,可溶性蛋白质及抗氧化酶活性明显增加,且随处理浓度增加均呈现先升后降的规律,随处理时间的有所增加。综合表明,2.5mmo1·L~(-1)的甜菜碱处理10d为缓解氧化损伤效果的最佳处理,此外100mL·L~(-1)的腐植酸、0.25mmo1·L~(-1)的SNP也有效地缓解了麻黄干旱胁迫,3种外源物质不同程度地提高了麻黄幼苗的抗旱能力,为干旱半干旱地区麻黄的推广栽培提供了依据。
Ephedra sinica was used as test material. The effects of humic acid, exogenous sodium nitroprusside (SNP) and betaine on the content of soluble protein, malondialdehyde (MDA) content and antioxidant enzyme activity changes. The results showed that under drought stress, the content of MDA in ephedra seedlings increased significantly, and the content of soluble protein and the activity of peroxidase (POD) and catalase (CAT) and the activity of superoxide dismutase (SOD) Under drought stress, the content of MDA decreased obviously and the content of soluble protein and antioxidant enzyme increased obviously with the increase of treatment concentration, and then increased with the increase of treatment concentration, and then increased with the treatment time. The results showed that treatment with 2.5 mmol·L -1 of betaine for 10 days was the best treatment to alleviate the oxidative damage. In addition, 100 mL·L -1 humic acid, 0.25 mmol·L -1 SNP also effectively alleviated the drought stress of Ephedra, and the three exogenous substances improved the drought resistance ability of Ephedra sinica to varying degrees, which provided the basis for the popularization and cultivation of Ephedra in arid and semi-arid areas.