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为研究中药有效成分天花粉蛋白质（ＴＣＳ）与苦瓜子蛋白质（ ＭＭＣ）的生物学活性，以 细胞遗传物质ＤＮＡ为底物，经分子生物学技术分析，发现超盘旋双链ＤＮＡ发生解旋和链结，单链 ＤＮＡ发生断链，在合适条件下，已解旋和断链的分子还可重新盘绕，再成超盘旋，极微量的蛋白质 即表现出很强的作用。这一活性与１型ＤＮＡ拓扑异构酶相同。
In order to study the biological activities of TCS and MMC, an effective component of traditional Chinese medicine, DNA was used as a substrate for molecular analysis of molecular biology techniques to reveal the unwinding and linking of double-stranded DNA. The single-stranded DNA breaks down. Under the right conditions, the untwisted and fragmented molecules can be re-coiled and then re-circulated. A very small amount of protein shows a strong effect. This activity is the same as type 1 DNA topoisomerase.