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目的:优选密闭性中药复合敷料的提取工艺。方法:以人参皂苷Rg1,Rb1含量和浸膏得率为评价指标,通过单因素试验优选提取次数,利用正交试验考察乙醇体积分数、乙醇用量、提取时间对密闭性中药复合敷料提取工艺的影响。采用HPLC测定人参皂苷Rg1,Rb1含量,流动相水(A)-乙腈(B)梯度洗脱(0~10 min,81%A;10~38 min,81%~79%A;38~90min,79%~66%A),检测波长203 nm。结果:各因素对提取工艺的影响顺序为乙醇体积分数>提取时间>乙醇用量。最佳提取工艺条件为加8倍量70%乙醇提取2次,每次2 h;平均浸膏得率25.45%,人参皂苷Rg1,Rb1平均质量浓度分别为0.46,0.37 g·L-1。结论:建立的含量测定方法准确、重复性好,优选的提取工艺稳定、合理、可行,为民间外伤验方的二次开发提供参考。
Objective: To optimize the extraction process of hermetically sealed traditional Chinese medicine composite dressing. Methods: Taking ginsenoside Rg1, Rb1 content and extract yield as evaluation index, the optimal extraction times were determined by single factor test. The effects of ethanol volume fraction, ethanol dosage and extraction time on the extraction process of compound Herbal Medicine dressing were studied by orthogonal test . The contents of ginsenosides Rg1 and Rb1 were determined by HPLC. The mobile phase consisted of gradient elution of water (A) -acetonitrile (B) (0-10 min, 81% A, 10-38 min, 81-79% A, 38-90 min, 79% ~ 66% A), detection wavelength of 203 nm. Results: The order of the factors affecting the extraction process was ethanol volume fraction> extraction time> ethanol consumption. The optimal extraction conditions were extracted twice with 8 times amount of 70% ethanol for 2 h, and the average extraction yield was 25.45%. The average concentrations of ginsenoside Rg1 and Rb1 were 0.46 and 0.37 g · L-1, respectively. CONCLUSION: The established assay method is accurate and reproducible. The optimal extraction process is stable, reasonable and feasible. It provides a reference for the secondary development of folk trauma prescription.