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目的:建立视神经不完全损伤动物模型。方法:实验动物分A、B、C3组,以动脉瘤夹临时阻断夹直接钳夹致伤视神经,各组钳夹时间分别为10s、30s和60s,伤后检测闪光刺激视觉诱发电位(F-VEP)、视神经组织切片和视网膜节细胞计数。结果:F-VEPP1波:A组伤后1d波幅上升,第14d和第28d波幅逐渐下降,潜伏期逐渐延长;B组伤后1d和第14d波幅进行性下降,潜伏期延长,第28d电活动消失;C组伤后第1d波幅下降潜伏期延长,第14d和第28均未检测到电活动。伤后7d视神经组织切片:A、B、C组视神经纤维变性比例分别为约30%、50%和70%。视网膜节细胞计数:A、B、C组伤后14d分别比正常减少37.4%、50.1%、55.9%,伤后28d分别比正常减少46.5%、65.2%、65.4%。结论:动脉瘤夹临时阻断夹直接钳夹视神经30s,可建立较好的视神经不完全损伤模型。
Objective: To establish an animal model of incomplete optic nerve injury. Methods: The experimental animals were divided into groups A, B and C3, and the optic nerve was injured by clipping the clip directly with the aneurysm clip. The clamping time of each group was 10s, 30s and 60s, respectively. After the injury, the visual evoked potentials (F -VEP), optic nerve tissue sections and retinal ganglion cell count. Results: The amplitude of F-VEPP1 wave increased at 1 day and the amplitude decreased gradually at 14th and 28th days. The latency of 1th and 14th days in group B decreased progressively, the latency prolonged and disappeared on the 28th day. In group C, the latency of decreasing amplitude on the 1st day after injury was prolonged, and no electrical activity was detected on the 14th and 28th days. Seven days after injury, optic nerve tissue sections: The optic nerve fiber degeneration ratio of group A, B and C were about 30%, 50% and 70% respectively. The number of retinal ganglion cells was decreased by 37.4%, 50.1% and 55.9% respectively at 14d after injury in group A, B and C, and decreased by 46.5%, 65.2% and 65.4% respectively than normal after 28 days. Conclusion: Aneurysm clip can directly occlude the optic nerve for 30s with a temporary occlusion clip, and a better model of incomplete optic nerve injury can be established.