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为明确大豆对灰斑病菌15号小种的抗性位点,以大豆抗病品种垦丰16、感病品种绥农10及其杂交F2、F3代群体为试验材料,在接种鉴定的基础上,运用SSR标记技术及分离群体组群分析法(BSA法)对垦丰16抗病基因进行了定位,并应用108份大豆新品系对标记进行了符合性检测。结果表明,垦丰16对15号小种的抗性受1对显性基因控制,抗病基因位于大豆染色体组的J连锁群上,将该基因定名为Rcs15。用Mapmaker/Exp 3.0 b进行连锁分析,获得了5个与抗病基因紧密连锁的SSR标记:Satt 529、Satt 431、Sat_151、Satt 547和Sat_224,标记与抗病基因间的排列顺序和遗传距离为Sat_151-10.7 cM-Satt 529-18.5 cMR-cs15-6.7 cM-Satt 547-7.8 cM-Sat_224-10.7 cM-Satt431。标记符合性检测结果显示,Satt 547和Sat_224的检测准确率达到85%以上,可用于分子标记辅助选择育种和抗源筛选。
In order to clarify the resistance loci of soybean to Cercospora sojae 15, Kenfeng 16, a susceptible cultivar of Suinong 10, F2 and F3 generations of susceptible cultivar Suinong 10, were selected as test materials. On the basis of inoculation identification , SSR markers and segregation population group analysis (BSA method) were used to locate Kenfeng 16 disease resistance genes, and 108 soybean lines were tested for compliance with the marker. The results showed that the resistance of Kenfeng 16 to race 15 was controlled by one dominant gene, and the resistance gene was located on J linkage group of soybean genome. The gene was designated as Rcs15. Using Mapmaker / Exp 3.0b, five SSR markers, Satt 529, Satt 431, Sat_151, Satt 547 and Sat_224, which were closely linked to disease resistance genes, were obtained. The sequence and genetic distance between the marker and the resistance gene were Sat_151-10.7 cM-Satt 529-18.5 cMR-cs15-6.7 cM-Satt 547-7.8 cM-Sat_224-10.7 cM-Satt431. Marker conformance test results showed that the detection accuracy of Satt 547 and Sat_224 was over 85%, which could be used for molecular marker-assisted selection breeding and resistance screening.