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目的:采用HPLC法测定复方甘草酸苷胶囊3组分的含量。方法:甘草酸苷:用ODS-C_(18)柱(150 mm×4.6 mm,5μm);2%冰醋酸-乙腈(65:35)为流动相,流速为1.0 ml·min~(-1),检测波长为252 nm;甘氨酸和蛋白酸:采用2,4-硝基氟苯柱前衍生化方法,色谱柱ODS C_(18)(250 mm×4.6 mm,5μm);乙腈-0.05 mol·L~(-1)醋酸钠缓冲液(35:65),检测波长:360 nm,流速1.0 ml·min~(-1)。结果:甘草酸苷、甘氨酸和蛋氨酸的线性范围分别为103.3～826.7μg·ml~(-1)(r=0.999 8),6.25～50.0μg·ml~(-1) (r=0.999 5)和6.33～50.67μg·ml~(-1)(r=0.999 5),其回收率分别为99.8%,99.7%和99.8%,RSD分别为0.3%,0.3%和0.4 (n=9)。结论:该法简便、灵敏、准确。
Objective: To determine the content of 3 components in compound glycyrrhizin capsules by HPLC. METHODS: Glycyrrhizin was eluted with ODS-C 18 column (150 mm × 4.6 mm, 5 μm) and 2% glacial acetic acid-acetonitrile (65:35) at a flow rate of 1.0 ml · min -1 , The detection wavelength was 252 nm; glycine and protein acid were obtained by column derivatization with 2,4-nitrofluorobenzene, column ODS C18 (250 mm × 4.6 mm, 5 μm), acetonitrile-0.05 mol·L ~ (-1) sodium acetate buffer (35:65). The detection wavelength was 360 nm and the flow rate was 1.0 ml · min ~ (-1). Results: The linear ranges of glycyrrhizin, glycine and methionine were 103.3-826.7μg · ml -1 (r = 0.999 8), 6.25-50.0μg · ml -1 (r = 0.999 5) and The recoveries were between 6.33 and 50.67 μg · ml -1 (r = 0.999 5). The recoveries were 99.8%, 99.7% and 99.8%, respectively. The RSDs were 0.3%, 0.3% and 0.4 (n = 9). Conclusion: This method is simple, sensitive and accurate.