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目的采用血管内弹簧圈置入法初步建立恒河猴可逆性大脑中动脉阻塞模型。方法选择4只成年健康恒河猴为实验动物,采用血管内介入技术,将1枚弹簧圈(2 mm×10 cm)置入大脑中动脉(MCA)起始部(不解脱弹簧圈),完全阻断MCA血流2 h,回收弹簧圈,恢复MCA血流灌注,初步建立恒河猴可逆性大脑中动脉阻塞模型。通过DSA、磁共振血管造影(MRA)、MRI及神经功能缺损情况评价动物模型是否成功。结果成功将弹簧圈置入4只恒河猴MCA起始部。弹簧圈释放后,MCA血流被完全阻断,远端血管不显影。阻断2 h,回收弹簧圈后MCA血流恢复。阻塞后2 h MRI检查显示,4只动物T1和T2加权相均未显示有梗死病灶,皮质和基底核区在弥散加权相(DWI)上出现异常高信号,MCA阻塞侧大脑半球肿胀。MRA检查显示MCA完全闭塞。动物苏醒后出现肢体偏瘫、口角歪斜等神经功能缺损症状。结论利用血管内弹簧圈栓塞技术制作恒河猴可逆性MCA阻塞模型具有可行性。
Objective To establish a model of reversible middle cerebral artery occlusion in rhesus monkeys by intravascular coil insertion. Methods Four adult healthy rhesus monkeys were selected as experimental animals. One endoplasmic reticulum (2 mm × 10 cm) was placed in the middle of the middle cerebral artery (MCA) by endovascular intervention Blocking MCA blood flow for 2 h, recovering the coil, restoring MCA perfusion and establishing a model of reversible middle cerebral artery occlusion in rhesus monkey. Animal models were evaluated for success by DSA, magnetic resonance angiography (MRA), MRI, and neurological deficits. Results The coils were successfully placed into the MCA start of 4 rhesus monkeys. After the coil was released, MCA blood flow was completely blocked and the distal blood vessels were not developed. Blocked 2 h, MCA recovery after recovery of blood flow. MRI examination at 2 h after occlusion showed that infarct lesions were not found in T1 and T2 weighted images of all four animals, abnormally high signals were observed in diffusely weighted phase (DWI) of the cortex and basal ganglia, and swelling of the MCA occluded hemisphere was observed. MRA showed complete MCA occlusion. After the animals wake up limb hemiplegia, skewness and other neurological deficit symptoms. Conclusion It is feasible to use the intracapsular coil embolization technique to make RBC reversible MCA occlusion model.