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目的观察结缔组织生长因子(CTGF)和转化生长因子β1(TGF-β1)在常染色体显性遗传性多囊肾(ADPKD)肾组织中的表达,探讨ADPKD的发病机制和可能的治疗靶点。方法采用免疫组织化学SABC染色法,分别检测CTGF和TGF-β1在67例ADPKD患者肾组织(ADPKD组)和24例正常肾组织(对照组)中的表达情况,分析两指标在ADPKD组表达的关系。结果 CTGF与TGF-β1在ADPKD组肾组织中的阳性表达率分别为86.6%(58/67)、83.6%(56/67),在对照组分别为54.2%(13/24)、62.5%(15/24),两两比较P均<0.05;CTGF与TGF-β1在ADPKD组肾组织中的表达呈正相关,r=0.701(P<0.05);CTGF在ADPKD组术侧肾功能代偿和失代偿者的阳性表达率分别为75.7%(28/37)、96.7%(29/30),在术侧肾脏体积≤750 cm3和>750 cm3者分别为76.5%(26/34)、97.0%(32/33),两两比较P均<0.05。结论 CTGF和TGF-β1在ADPKD肾脏组织中高表达,两者可能在囊肿的发生、发展和肾间质纤维化过程中具有重要协同作用;以CTGF为治疗靶点可能成为减轻ADPKD肾组织纤维化进展的新途径。
Objective To observe the expression of connective tissue growth factor (CTGF) and transforming growth factor-β1 (TGF-β1) in autosomal dominant polycystic kidney disease (ADPKD) and to explore the pathogenesis of ADPKD and possible therapeutic targets. Methods Immunohistochemical SABC staining was used to detect the expression of CTGF and TGF-β1 in 67 cases of ADPKD and 24 cases of normal renal tissues (control group) respectively. The expressions of two markers in ADPKD group relationship. Results The positive rates of CTGF and TGF-β1 in renal tissue of ADPKD group were 86.6% (58/67) and 83.6% (56/67), respectively, which were 54.2% (13/24) and 62.5% (P <0.05); CTGF and TGF-β1 expression in renal tissue of ADPKD group was positively correlated, r = 0.701 (P <0.05); CTGF in ADPKD group renal side function compensation and loss The positive rates of compensators were 75.7% (28/37) and 96.7% (29/30), respectively, with 76.5% (26/34) and 97.0% of patients with renal volume ≤750 cm3 and> 750 cm3 respectively (32/33), any two comparisons P <0.05. Conclusions CTGF and TGF-β1 are highly expressed in renal tissues of ADPKD patients, both of which may play an important synergistic role in the occurrence and development of cysts and in the process of renal interstitial fibrosis. CTGF as a therapeutic target may reduce the progression of renal fibrosis in ADPKD The new way.