传统的LD_(50)试验是Trevan首先提出的。这一方法一直被毒理学家接受,并被许多管理机构所采用,作为化学物急性毒性分级的依据。但是,自80年代以来,有人对其提出了异议。认为LD_(50不是生物常数而是一个统计量,同一化学物在同一品系动物、同样的接触途经、在不同的实验 The traditional LD ​​50 test was first proposed by Trevan. This method has been accepted by toxicologists and adopted by many regulatory agencies as the basis for the classification of acute toxicity of chemicals. However, there have been objections to it since the 1980s. Considering LD_ (50) is not a biological constant but a statistic, the same chemical in the same strain of animals, the same contact through, in different experiments