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目的：了解不同年龄段健康孕妇25-OH-总维生素D(25-OHD)的水平分布。方法选择孕12周及28周健康孕妇,按照年龄分为4组,分别为35.1岁组。检测当天留取空腹静脉血,采用电化学免疫发光法对标本进行25羟基维生素D(25-OHD)的检测,并对结果进行统计学分析。结果4321例健康孕妇的25-OHD的平均水平为16.21 ng/mL,各个年龄段25-OHD水平均属25-OHD不足或缺乏状态,>35.1岁组尤其明显。35.1岁组25-OHD含量严重缺乏的人数比例最大,为58.9%。分别对4组的25-OHD含量充足、不足、缺乏、严重缺乏的人数进行比较,采用多组等级资料的χ2检验,结果差异有统计学意义(P 35. 1 age group especial y, the 35. 1 age group 25 OHD content seriously lack of the percentage of maximum,is 58. 9%. The number of 25OHD content respectively 4 groups enough, insuf icient, lack,lack of comparison,χ2 test using multiple groups of rank data, results there were signifi-cant dif erences(P <0. 05). Conclusion More than 90% of pregnant women, vitamin D content are inadequate or lack evena serious lack of situation, Aged more than 35 years had a serious lack of vitamin D is particularly significant,should cause enough at ention , And the 25OHD levels in pregnant women and early screening and intervention to improve maternal and fetal health expectations.