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为了研究乙烯在西瓜(Citrullus lanatusThunb.Mansfeld)果实水渍化败坏过程中的作用,先将果实在5μL/L 1-甲基环丙烯(1-MCP)气体中处理18 h,然后在50 μL/L乙烯和20℃温度下贮藏。西瓜果实对乙烯处理的最初反应表现为胎座组织的电导率和游离汁液增加,同时出现组织软化和水渍化。水渍化的症状最初在靠近花萼端的内果皮中发生,在乙烯处理的第2天开始出现,ACC合成酶(ACS)和ACC氧化酶(ACO)的活性明显提高。1-MCP单独处理不产生任何明显的作用,但是会完全抑制外源乙烯诱导的水渍化败坏。没有经过 乙烯处理的西瓜果实,贮藏2 d以后出现呼吸强度和乙烯释放量的高峰,10 d以后水渍化现象也零星出现。这些结果和1-MCP的预防效果说明,西瓜果实的水渍化败坏是一种由乙烯诱导的衰老现象。
In order to study the role of ethylene in the watermelon degradation of watermelon (Citrullus lanatus Dunbansun), the fruit was treated for 18 h with 5 μL / L 1- methylcyclopropene (1-MCP) gas and then incubated at 50 μL / L ethylene and stored at 20 ° C. The initial reaction of watermelon fruit to ethylene treatment showed an increase in the conductivity and free juice of the placental tissue with tissue softening and water stains. The symptoms of waterlogging first occurred in the endocarp near the calyx end, appearing on the second day of ethylene treatment, and the activities of ACC synthase (ACS) and ACC oxidase (ACO) were significantly increased. 1-MCP alone did not produce any significant effect, but completely inhibited exogenous ethylene-induced water damage. Watermelon fruits without ethylene treatment showed the peak of respiration intensity and ethylene emission after storage for 2 days, and the phenomenon of waterlogging appeared sporadically after 10 days. These results and the prophylactic effect of 1-MCP suggest that watermelon spoilage of watermelon fruit is a senescence-induced phenomenon by ethylene.