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采用ISSR分子标记对浙江七叶一枝花8个野生种源地的共48个个体进行遗传多样性分析。结果显示:用13个随机引物共扩增出90条清晰条带,其中79条具多态性,平均多态位点百分率(PPL)为87.78%,Nei’s基因多样度He值为0.165 9,Shannon信息指数I值为0.240 1,遗传分化系数Gst为0.442 2。浙江野生七叶一枝花资源具有丰富的遗传多样性,种源内的遗传相似性高于种源间,资源分布具有明显的地域特征。
ISSR markers were used to analyze the genetic diversity of a total of 48 individuals from 8 wild provenances of Aescin from Zhejiang Province. The results showed that 90 clear bands were amplified by 13 random primers, of which 79 were polymorphic, the mean percentage of polymorphic loci (PPL) was 87.78%, the Nei’s gene diversity He value was 0.165 9, Shannon The information index I value is 0.240 1, and the genetic differentiation coefficient Gst is 0.442 2. Zhejiang wild seven-leaf flower resource has rich genetic diversity, the genetic similarity within the provenance is higher than between provenances, resource distribution has obvious geographical features.