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目的了解IL-1F10-3基因rs3811058位点的单核苷酸多态性与强直性脊柱炎遗传易感性的关系。方法采用病例对照研究设计,通过聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对基因型筛查并进行基因组测序验证,检测151例强直性脊柱炎患者和155例正常对照基因型,并分析基因型及等位基因频率在两组中的分布。结果 IL-1F10-3基因rs3811058位点的等位基因频率和基因型频率在病例组和对照组中的分布差异均无统计学意义。结论安徽地区汉族人群强直性脊柱炎患者遗传易感性可能与IL-1F10-3基因rs3811058位点单核苷酸多态性无关。
Objective To understand the relationship between SNP rs3811058 in IL-1F10-3 gene and genetic predisposition to ankylosing spondylitis. Methods A case-control study was designed. Genotypes were screened by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and verified by genome sequencing. 151 patients with ankylosing spondylitis and 155 normal controls Genotype, and analysis of genotypes and allele frequencies in both groups. Results There was no significant difference in allele frequency and genotype frequency between IL-1F10-3 rs3811058 locus and control group. Conclusion The genetic susceptibility to ankylosing spondylitis in Han nationality in Anhui province may not be related to the single nucleotide polymorphism of rs3811058 in IL-1F10-3 gene.