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耶尔森氏菌外膜蛋白（Ｙｏｐｓ）在菌体中所占比例甚小，因而提取与纯化均有一定难度，国内尚无成功的先例。我们使用了一种盐析加电泳的方法提取了Ｙｏｐ１蛋白。实验结果表明，该法简便、易行且重复性好、回收率高。使用本法提取假结核耶氏菌外膜蛋白１（Ｙｏｐ１），自每升培基增殖的菌体中（约４．５ｇ湿重），可获得纯品６．３ｍｇ，回收率高于国外同类研究的６倍以上［１］。一次性提取的Ｙｏｐ１蛋白在十二烷基磺酸钠－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）中共显示出三条可辩认的蛋白带，其分子量依次为１５０ＫＤ，９０～１００ＫＤ及４５～５０ＫＤ。进一步的尿素－十二烷基磺酸钠－聚丙烯酰胺凝胶电泳结果表明，样品中的约１００ＫＤ和５０ＫＤ两种蛋白实际为１５０ＫＤ蛋白解聚后的二聚体和单体，根据这一结果，推测该种蛋白系三聚体，其单体分子量为４５～５０ＫＤ。
Yersinia outer membrane protein (Yops) in the proportion of bacteria is very small, so extraction and purification have a certain degree of difficulty, there is no precedent for the success of the country. We used a salting-out / electrophoresis method to extract the Yop1 protein. The experimental results show that the method is simple, easy and reproducible, and high recovery rate. This method was used to extract Yop1, the pure product of 6.3 mg was obtained from the proliferated bacterial cells (about 4.5 g wet weight) per liter of culture medium. The recovery rate was higher than that of foreign similar More than 6 times the study [1]. Yop1 protein extracted at a time showed a total of three identifiable protein bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with molecular weights of 150KD, 90-100KD and 45-50KD . Further urea-sodium dodecyl sulfate-polyacrylamide gel electrophoresis results show that the two proteins of about 100 KD and 50 KD in the sample are actually dimers and monomers after 150 KD protein depolymerization. Based on this result , Suggesting that the protein trimer, the monomer molecular weight of 45 ~ 50KD.