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[目的]用鸭乙型肝炎(乙肝)模型研究肝康栓抗鸭乙型肝炎病毒(DHBV)和改善肝脏病理的作用。[方法]用DHBV阳性血清感染1 d龄的樱桃谷雏鸭,制备鸭乙肝模型。将其中36只感染阳性鸭随机分成3组:肝康栓治疗组,0.85%氯化钠模型组和阿昔洛韦(ACV)对照组,每组12只,均连续给药4周。分别于给药前,给药14、28 d和停药7 d时取血清,用实时定量PCR法检测鸭血清中DHBV DNA拷贝数。于停药7 d时,处死各组雏鸭,取肝脏病理切片苏木精-伊红染色后,观察肝脏炎症情况和肝细胞变性程度。[结果]肝康栓治疗组给药14、28 d及停药7 d时鸭血清中DHBV DNA拷贝数的对数值均较给药前降低(均P<0.01)。肝康栓治疗组雏鸭肝脏的肝细胞肿胀率、空泡变率、嗜酸性变率均较同期模型组降低,差异有统计学意义(P<0.05,<0.01)。[结论]肝康栓能有效地抑制鸭体内DHBV DNA的复制,并具有改善肝脏病理的作用。
[Objective] To study the effect of Gankang suppository against duck hepatitis B virus (DHBV) and improve liver pathology with duck hepatitis B (hepatitis B) model. [Methods] A duckling hepatitis B model was prepared by infecting 1 year old Cherry Valley ducklings with DHBV positive serum. Thirty-six of the infected ducks were randomly divided into three groups: the liver Kangshuan treatment group, the 0.85% sodium chloride model group, and the acyclovir (ACV) control group, each of which was administered continuously for 4 weeks. Serum samples were taken before and 14d, 28d, and 7d withdrawal, and the DHBV DNA copy number in duck serum was detected by real-time quantitative PCR. At 7 days after discontinuation, ducklings from each group were sacrificed and hepatic pathological sections were stained with hematoxylin-eosin to observe the hepatic inflammation and hepatocyte degeneration. [Results] The logarithm of DHBV DNA copy number in the serum of the liver Kangshuan treatment group at the 14th, 28th day and 7th day after discontinuation was lower than that before the administration (all P<0.01). The hepatocyte swelling rate, vacuolization rate, and eosinophilia rate of the liver of the ducklings in the treatment group were all lower than those in the model group at the same time (P<0.05, <0.01). [Conclusion] Gankang suppository can effectively inhibit the duplication of DHBV DNA in ducks and has the effect of improving liver pathology.