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利用亲和层析纯化的抗包虫免疫球蛋白以双抗体ELISA法检测包虫抗原,对经亲和层析纯化的包虫抗原的检出下限可达40ng/ml。以1:2健康人混合血清稀释抗原时,检出下限为2.5μg/ml。用此法检测79例包虫病人的循环抗原,阳性率为40.51％。其中肝包虫病人阳性率为38.98％,肺包虫病人为33.33％。血清抗体阳性的包虫病人循环抗原检出率为33.33％,抗体阴性者中为43.75％。在抗体阴性的肺包虫病人中循环抗原的阳性率可达50％。循环抗原的测定在血清抗体阴性包虫病人的免疫学诊断中具有辅助意义。
Anti-hydatid immunoglobulin purified by affinity chromatography was used to detect hydatid antigen by double-antibody ELISA. The detection limit of hydatid antigen purified by affinity chromatography was up to 40ng / ml. When diluted 1: 2 healthy human serum diluted antigen, the detection limit of 2.5μg / ml. 79 cases of hydatidosis were detected by this method, the positive rate was 40.51%. Among them, the positive rate of liver hydatid disease was 38.98% and that of lung hydatid disease was 33.33%. Serum antibody-positive echinococcosis patients detected 33.33% of circulating antigen, antibody-negative were 43.75%. In antigen-negative pulmonary hydatid disease in patients with circulating antigen positive rate of up to 50%. Determination of circulating antigen in serum antibody negative hydatid immunocompromised in patients with secondary meaning.