目的:建立尿感宁颗粒(无糖型)简单、快捷的质量控制方法。方法:采用薄层色谱法对尿感宁颗粒中的连钱草、凤尾草进行定性鉴别,用高效液相色谱法对尿感宁颗粒中秦皮乙素的含量进行测定,色谱柱为C18柱(4.6 mm×250 mm,5μm),流速为1.0 mL.min-1,流动相为甲醇-0.5%磷酸(28∶72),检测波长为345 nm。结果:连钱草、凤尾草和紫花地丁薄层色谱分离良好,斑点清晰可见;含量测定中秦皮乙素的含量在20.08～2 080 ng内线性关系良好,平均加样回收率为96.3%,RSD1.9%,n=6。结论:方法结果准确、重复性好,可作为该制剂的质量控制方法。 Objective: To establish a simple and quick method of quality control of urinary sensitive granules (sugar-free type). Methods: The TLC and HPLC method was used to identify the contents of aesculetin in urine granules of Cinnabaris and Pteris by HPLC. The content of aesculetin in urine granules was determined by C18 column (4.6 mm × 250 mm, 5 μm) at a flow rate of 1.0 mL · min-1. The mobile phase consisted of methanol-0.5% phosphoric acid (28:72) and the detection wavelength was 345 nm. Results: TLC, TLC and HPLC were well separated and the spots were clearly visible. The content of aesculetin in the determination ranged from 20.08 to 2.080 ng, and the average recovery was 96.3% , RSD 1.9%, n = 6. Conclusion: The method is accurate and reproducible, which can be used as quality control method of the preparation.