【摘 要】
：
The transcripts of beet necrotic yellow virus RNA5 were prepared from the cloned cDNA in a bacteriophage T7 in vitro run-off transcription system. The RNA5 tran
【机 构】
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State Key Laboratory for Agrobiotechnology and College of Biological Science
【基金项目】
：
国家高技术研究发展计划(863计划)
论文部分内容阅读
The transcripts of beet necrotic yellow virus RNA5 were prepared from the cloned cDNA in a bacteriophage T7 in vitro run-off transcription system. The RNA5 transcripts and RNAs extracted from two BNYVV mutants, Hu0 containing RNA1, 2 and Hu3 containing RNA1, 2, 3 respectively, were co-inoculated to Tetragonia expansa and sugar beet seedlings. The results indicated that the virus infections and accumulation in the hosts could be improved by presence of RNA5. Cooperating with RNA3, the RNA5 seems to cause more severe symptom and yield losses in sugar beet. These results suggest that, in addition of RNA3, BNYVV RNA5 is another important factor associated with the virus pathogenicity.
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