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目的:探讨肿瘤坏死因子-α(TNF-α)基因启动子-308G/A多态性与豫南地区汉族人群类风湿性关节炎(RA)的相关性。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析90例RA患者及100例正常对照TNF-α基因启动子-308G/A多态性。结果:RA患者与正常对照相比,-308基因型频率和等位基因频率差异无统计学意义(P均>0.05);RA患者组中有侵蚀性病变患者-308位点G等位基因频率与无侵蚀性病变患者相比差异有待进一步研究(χ2=4.074,P=0.044)。结论:TNF-α基因启动子-308G/A多态性可能与豫南地区汉族人群RA无关,但可能与关节的侵蚀性病变有关。
Objective: To investigate the relationship between the -308G / A polymorphism of tumor necrosis factor-α (TNF-α) gene promoter and rheumatoid arthritis (RA) in Han population of southern Henan. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the polymorphism of TNF-α gene promoter -308G / A in 90 RA patients and 100 normal controls. Results: There was no significant difference in the frequencies of -308 genotypes and alleles between RA patients and normal controls (all P> 0.05). The frequency of G allele at -308 in RA patients with aggressive lesions Compared with non-erosive lesions in patients to be further studied (χ2 = 4.074, P = 0.044). CONCLUSION: The -308G / A polymorphism of TNF-α gene promoter may not be associated with RA in Han population of southern Henan Province, but it may be related to the aggressive lesions of the joint.