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通过给予头孢曲松造成SPF级BALB/c小鼠(Mus musculus)发生肠道菌群紊乱,进而通过连续灌胃密度为2×107CFU/m L的白假丝酵母(Candida albicans)菌液以建立小鼠肠道真菌过增殖模型;通过镜检、活菌计数、结肠组织病理切片、电镜观察等方法评价白假丝酵母在小鼠体内黏附定植的情况。实验结果显示,大剂量头孢曲松处理后,活菌计数结果显示小鼠肠道菌群出现重度失衡;灌胃真菌后,镜检可见酵母相和菌丝相的白假丝酵母,PAS染色可见小鼠结肠粘膜表面黏附有大量染成红色的圆形真菌孢子;扫描电镜观察发现小鼠结肠表面黏附大量卵圆型白假丝酵母的酵母相细胞。研究认为大剂量抗生素处理结合真菌灌胃,可成功建立小鼠肠道真菌过增殖模型。
Intestinal microflora was disturbed in SPF-grade BALB / c mice (Mus musculus) by ceftriaxone administration, and then by continuous gavage of Candida albicans at a density of 2 × 10 7 CFU / mL to establish Mouse intestinal fungal overgrowth model; By Candida, viable count, colon histopathology, electron microscopy and other methods to evaluate Candida albicans adhesion in mice in vivo colonization. The experimental results showed that after the treatment with high dose of ceftriaxone, viable count results showed that there was a serious imbalance in the intestinal flora of mice. After fungi were inoculated, the Candida albicans yeast phase and mycelial phase were observed microscopically and the PAS staining was visible A large number of circular round fungal spores were stained on the surface of colonic mucosa of mice. Scanning electron microscopy showed that a large number of yeast phase cells adhered to Candida albicans on the colon surface of mice. The study concluded that high-dose antibiotic treatment combined with fungal gavage successfully established mouse intestinal fungal over-proliferation model.