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为研究壳寡糖处理对杏果实贮藏期间抗病作用中活性氧变化的影响。以新疆塞买提杏为试验材料,选用分子量5 000 u、浓度为0.50%的壳寡糖对杏果实进行减压渗透处理。处理后的杏果实贮藏在温度为4℃、RH 90%~95%的冷库中,定期测定杏果实接种发病率、病斑直径、超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)、过氧化物酶(Peroxidase,POD)、抗坏血酸过氧化物酶(Ascorbate peroxidase,APX)的活性和超氧阴离子(Superoxide anion,·)产生速率及过氧化氢(Peroxide,H2O2)的含量。结果表明,壳寡糖处理的杏果实发病率显著低于对照果实,且可诱导杏果实早期·和H2O2的含量的积累,并使·产生速率和H2O2含量在杏果实贮藏后期保持在较低水平,增强了杏果实中SOD、CAT、POD和APX的活性。说明壳寡糖处理可降低果实发病率,诱导活性氧的迸发和相关抗氧化酶酶活性的显著增强,提高了果实的抗病性。
In order to study the effect of chitooligosaccharides treatment on the changes of reactive oxygen species in the apricot fruit during storage. Apricot fruit was subjected to reduced pressure osmosis by using Cejeda as test material and chitosan oligosaccharide with molecular weight of 5,000u and concentration of 0.50%. The processed apricot fruits were stored in cold storage at 4 ℃ and 90% -95% RH. The apricot fruit incidence, the diameter of diseased spots, superoxide dismutase (SOD) and catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) activity and superoxide anion production rate and Peroxide (H2O2) Content. The results showed that the incidence of apricot fruit treated with chitooligosaccharides was significantly lower than that of the control fruits and could induce the accumulation of early apricot and H2O2 contents and keep the production rate and H2O2 content at a relatively low level , Enhance the activity of apricot fruit SOD, CAT, POD and APX. Chitosan oligosaccharide treatment can reduce the incidence of fruit, induced active oxygen burst and related antioxidant enzyme activity was significantly increased, and improve the fruit’s disease resistance.