蛋白质组是指特定细胞、组织或生物体在特定时间所表达的全部蛋白,对其进行分析的学科被称为蛋白质组学。在蛋白质组学的诸多研究方法中,出现最早且目前仍能提供蛋白质组分析所要求的高分辨率的唯一技术,是双向电泳(2-D PAGE),由于其可提高分辨率、重复性和简易性良好,已成为蛋白质组研究的核心技术。2-D PAGE用于蛋白质组蛋白表达的全面分析时,能够分离特定细胞和组织中成百上千的蛋白形式(包括翻译后的变异体)。本研究中,我们以 A proteome is the complete protein that a particular cell, tissue, or organism expresses at a particular time, and the discipline under which it is analyzed is called proteomics. Among the many proteomics research methods, the only technique that emerged the earliest and still offers the high resolution required for proteomic analysis is 2-D PAGE because of its ability to improve resolution, repeatability and Simplicity, has become the core technology of proteome research. When used in a comprehensive analysis of proteomic histone expression, 2-D PAGE enables the isolation of hundreds of protein forms (including translated variants) in specific cells and tissues. In this study, we take