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利用带有氯霉素乙酰转移酶报道基因的检测载体，从痘苗病毒基因组 Ｄ Ｎ Ａ 中筛选到一个增强子样片段。序列分析表明，该片段长２８３ｂｐ ，是痘苗病毒 Ｄ Ｎ Ａ 依赖性 Ｒ Ｎ Ａ 聚合酶的一部分，具有两段２４ｂｐ 长的重复序列。采用带有β－ 半乳糖甘酶报道基因的载体检测发现，该片段正向可以使报道基因表达增强１０９ 倍，反向可以增强３８ 倍。 Ｒ Ｎ Ａ ｄｏｔ ｂｌｏｔ 实验证实，它对基因的增强活性表现在转录水平上。
An enhancer-like fragment was screened from vaccinia virus genome DNA using a detection vector with a chloramphenicol acetyltransferase reporter gene. Sequence analysis showed that the fragment was 283 bp long and was a part of vaccinia virus D N A-dependent R N A polymerase with two 24 bp long repeats. Using beta-galactosidase reporter gene vector detection found that the positive fragment can make the reporter gene expression enhanced 10 9 times, the reverse can be enhanced 8 times. R N A dot blot experiments confirmed that its enhanced activity of the gene is expressed at the transcriptional level.