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目的:探讨子宫内膜异位症(EMs)在位内膜细胞凋亡特性的改变。方法:①应用免疫组化方法对32例EMs在位子宫内膜组织及24例对照组子宫内膜组织的Survivin蛋白及Ki-67蛋白的表达进行测定;②应用TUNEL技术监测两组子宫内膜细胞的凋亡特性。结果:EMs在位内膜组织Survivin蛋白表达高于对照组,差异有统计学意义(P<0.05);EMs在位内膜细胞分泌期凋亡指数低于同期对照组子宫内膜,差异有统计学意义(P<0.05);EMs在位内膜组织Ki-67蛋白表达与对照组差异无统计学意义(P>0.05)。结论:EMs在位子宫内膜细胞因凋亡能力下降并非高度增殖导致细胞活性升高,在位内膜细胞的生物学特性改变在EMs的病理生理中可能起着重要作用。
Objective: To investigate the changes of endometrial apoptosis in patients with endometriosis (EMs). Methods ① Immunohistochemistry was used to detect the expression of Survivin protein and Ki-67 protein in 32 cases of endometrial tissue and 24 cases of control group. ②TUNEL technique was used to detect the expression of endometrial Apoptosis characteristics of cells. Results: The expression of Survivin protein in eutopic endometrium of EMs was higher than that of control group (P <0.05). The apoptotic index of eutopic endometrial cells in EMs was lower than that of endometrium in control group (P <0.05). There was no significant difference in the expression of Ki-67 protein in EMs eutopic endometrium and the control group (P> 0.05). CONCLUSION: The decrease of apoptotic capacity of endometrial cells in EMs is not due to the high degree of proliferation, which leads to the increase of cell viability. The change of biological characteristics of endometrial cells may play an important role in the pathophysiology of EMs.