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比较了4种方法(结晶紫果胶酸钠培养基直接分离、富集分离技术、雾室技术和厌气技术)检查薯块带菌率的结果,平均10个薯块的带菌薯块数分别为1、7.3、8.7、10。用厌气技术对来自全国23个省、市和地区的46份样本中的460块薯块进行带菌率检查的结果,各样本之间的带菌率高低是不同的,在0～100%之间,平均为39.3%,即共有181块薯块由于软腐欧氏杆菌的危害而发生腐烂。从33份腐烂马铃薯样本中,共分离到284个软腐欧氏杆菌的菌株。鉴定结果:其中206个菌株(占72.5%)是Erwinia carotovora var.carotovora,发现于各省(区)所有马铃薯样本中;有15个菌株(占5.3%)鉴定为Erwinia ca-rotovora var.atroseptica,主要发现于北方省区(如黑龙江、青海、河北、甘肃以及四川等省);29个菌株(占10.2%)鉴定为Erwinia chrysanthemi,发现于来自四川、江苏、江西和内蒙的样本中。另有34个菌株,根据其细菌学性状分别归入两个不同类型的中间型,介于E.carotovora var.carotovora 和E.chrysanthemi 之间。
The results of four methods (direct separation of crystal purple pectate medium, enrichment and separation technology, fog chamber technology and anaerobic technique) were compared. The average number of tubers carrying 10 potato tubers was 1,7.3,8.7,10. Using anaerobic technique to check the carriage rate of 460 tubers in 46 samples from 23 provinces, cities and regions across the country, the rates of carriage of bacteria in each sample were different from 0 to 100% , An average of 39.3%, that is, a total of 181 pieces of potato due to the harm caused by soft rot of rotten rot. A total of 284 Staphylococcus aureus isolates were isolated from 33 rotten potato samples. The results showed that 206 strains (72.5%) were Erwinia carotovora var. Carotovora and were found in all the potato samples of each province (province); 15 strains (accounting for 5.3%) were identified as Erwinia ca-rotovora var. Atroseptica, Found in northern provinces (such as Heilongjiang, Qinghai, Hebei, Gansu and Sichuan provinces); 29 strains (10.2%) were identified as Erwinia chrysanthemi and were found in samples from Sichuan, Jiangsu, Jiangxi and Inner Mongolia. Another 34 strains were classified into two different types of intermediates according to their bacteriological traits, between E.carotovora var. Carotovora and E. chrysanthemi.