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目的考察温度与气泡对大孔吸附树脂纯化白芍中芍药苷的影响。方法以芍药苷为指标,采用Hypersil ODS(4.6mm×250 mm,5μm)色谱柱,以乙腈-0.1%(V/V)磷酸溶液(14∶86)为流动相,流速1.0 ml/min,检测波长为230 nm,柱温25℃,测定大孔吸附树脂在不同温度和有无气泡因素下芍药苷的含量。结果在40℃条件下,大孔吸附树脂对芍药苷比吸附量比室温下提高8.6%,而解吸附洗脱剂用量比室温下减少2倍量;树脂柱中有气泡与无气泡相比,大孔吸附树脂对芍药苷比吸附量下降6.4%;而比洗脱量下降3.2%。结论温度和树脂中气泡对大孔吸附树脂吸附和解吸附有很大影响,应引起足够重视。
Objective To investigate the effects of temperature and air bubbles on the purification of paeoniflorin from macroporous adsorption resin. Methods The paeoniflorin was used as an indicator to determine the content of paeoniflorin in Hypersil ODS column (4.6 mm × 250 mm, 5 μm) with acetonitrile-0.1% (V / V) phosphoric acid solution Wavelength of 230 nm, column temperature 25 ℃, determination of macroporous resin at different temperatures and with or without air bubbles paeoniflorin content. Results The adsorption capacity of macroporous resin to paeoniflorin increased by 8.6% at room temperature and the amount of desorption eluent was reduced by two times than that at room temperature under the condition of 40 ℃. Compared with no bubbles in the resin column, The macroporous adsorption resin decreased the specific adsorption of paeoniflorin by 6.4%, while the specific elution amount decreased by 3.2%. Conclusion The temperature and resin bubbles on macroporous resin adsorption and desorption have a great impact, should cause enough attention.