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目的建立高效液相色谱法测定万古霉素的血药浓度。方法血浆样品经硫酸锌沉淀处理,采用反相高效液相色谱法,流动相为乙腈-0.02moL·L-1磷酸二氢钾缓冲液(8∶92,V/V),检测波长为236nm,流速为1.0 mL·min-1,柱温为25℃,进样量为10μL。采用峰面积外标法定量测定人血浆中的万古霉素浓度。结果万古霉素进样浓度在2.5~200 mg·L-1范围内与峰面积积分值呈良好的线性关系(r=0.9997,n=7);日内RSD为3.91%,2.38%,1.77%,日间RSD为4.01%,2.35%,1.82%;相对回收率分别为99.2%~100.3%;稳定性考察结果表明血浆样品在24h内稳定。结论本方法专属性强,简单方便,适用于万古霉素的血药浓度监测。
Objective To establish a method for the determination of vancomycin in plasma by high performance liquid chromatography. Methods The plasma samples were treated with zinc sulfate precipitation. The mobile phase consisted of acetonitrile-0.02 mol L-1 potassium dihydrogen phosphate buffer (8:92, V / V) with reversed-phase high performance liquid chromatography The flow rate was 1.0 mL · min-1, the column temperature was 25 ℃ and the injection volume was 10μL. The peak area of external standard method for the quantitative determination of vancomycin in human plasma concentrations. Results The concentration of vancomycin in the range of 2.5-200 mg · L-1 showed a good linear relationship with the peak area (r = 0.9997, n = 7). The intraday RSD was 3.91%, 2.38% and 1.77% The daytime RSD was 4.01%, 2.35% and 1.82%, respectively. The relative recoveries were 99.2% ~ 100.3% respectively. The stability test results showed that the plasma samples were stable within 24h. Conclusion This method is specific, simple and convenient, suitable for the monitoring of vancomycin blood concentration.