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目的:观察消渴平合剂对链脲佐菌素(STZ)诱导的糖尿病肾病(DN)大鼠TGF-β1/Smad7信号转导通路的影响,探讨其对DN的防治作用及机制。方法:复制DN大鼠模型,随机分为正常组10只,模型组10只、厄贝沙坦组10只和消渴平合剂组10只。12周时处死大鼠,观察各组大鼠24 h尿蛋白变化情况,检测血清尿素氮(BUN)、血肌酐(Scr)、甘油三酯(TG)、一氧化氮(NO),大鼠肾组织行HE染色,免疫组化检测肾组织TGF-β1及Smad7蛋白。结果:消渴平合剂可以改善DN大鼠的一般状况,明显降低24 h尿蛋白定量(P<0.01),减轻肾组织病理性损害。与模型组比较,消渴平合剂组BUN、Scr、TG含量显著降低(P<0.01),NO含量明显升高(P<0.01);肾组织TGF-β1蛋白表达明显降低(P<0.01),Smad7蛋白表达显著升高(P<0.01)。结论:消渴平合剂可用于治疗糖尿病肾病,其机制可能与抑制DN大鼠肾脏TGF-β1/Smad7信号转导通路的激活有关。
Objective: To observe the effect of Xiaokepingheji on TGF-β1 / Smad7 signal transduction pathway induced by streptozotocin (STZ) in diabetic nephropathy (DN) rats and its preventive and therapeutic effects on DN. Methods: DN model rats were duplicated and randomly divided into normal group (n = 10), model group (n = 10), irbesartan group (n = 10) and Xiaokeping agent group (n = 10). The rats were sacrificed at 12 weeks, and the changes of 24 h urinary protein in each group were observed. Serum urea nitrogen (BUN), serum creatinine (Scr), triglyceride (TG), nitric oxide Tissues were stained with HE and immunohistochemically examined for TGF-β1 and Smad7 protein in renal tissues. Results: Xiaokeping mixture can improve the general condition of DN rats, significantly reduce 24 h urinary protein (P <0.01) and reduce the pathological damage of renal tissue. The levels of BUN, Scr and TG in Xiaokeping Mixture group were significantly lower than those in model group (P <0.01), and the contents of NO were significantly increased (P <0.01) Smad7 protein expression was significantly increased (P <0.01). Conclusion: Xiaokeping Mixture can be used to treat diabetic nephropathy, which may be related to the inhibition of TGF-β1 / Smad7 signal transduction pathway in the kidney of DN rats.