论文部分内容阅读
目的 :探讨微波作为一种非电离辐射波 ,对睾丸间质细胞 NOS活性的影响 ,以进一步深入研究微波对睾丸的损伤效应。方法 :利用 NADPH-d黄递酶组织化学方法显示睾丸间质细胞 NOS活性变化。结果 :2 1天频率为 2 .45 GHz,功率密度为 2 m W/ cm2 和 5 m W/ cm2 的连续微波辐射实验组与假辐照组相比 ,N OS阳性睾丸间质细胞的数量无明显变化 ,仅着色稍变淡 ;而以上剂量 42天微波辐射后 ,两微波实验组的睾丸间质细胞 NOS阳性睾丸间质细胞在明显减少的同时 ,染色也变淡。结论 :睾丸间质细胞 NOS活性与微波辐射强度密切相关。提示 NO机制可能是微波对生精过程影响的机制之一。
Objective: To investigate the effect of microwave as a kind of non-ionizing radiation wave on the activity of NOS in testicular stromal cells, so as to further study the damage effect of microwave on testis. Methods: NADPH-d diaphorase histochemical method was used to show the changes of NOS activity in testicular stromal cells. Results: Compared with sham irradiation group, the number of NOS-positive testicular stromal cells in 21 experimental groups with 2 days frequency of 2.45 GHz and power densities of 2 mW / cm2 and 5 mW / cm2 Obvious change, only slightly lighter; and 42 days after the above dose of microwave radiation, two experimental group of testicular stromal cells of NOS-positive testicular stromal cells was significantly reduced at the same time, staining also fades. Conclusion: The activity of NOS in testicular stromal cells is closely related to the intensity of microwave radiation. Suggesting that the NO mechanism may be one of the mechanisms by which microwaves affect the spermatogenesis process.