论文部分内容阅读
目的 探讨体液中检测膀胱癌肿瘤细胞DNA的方法和意义。方法 应用聚合酶链反应 (PCR)扩增 30例膀胱癌患者血浆和尿液中p53基因第 5、6、7和 8外显子片断 ,应用单链构象多态分析 (SSCP)法检测变异 ,并对变异片断测序。对照组织中的检测结果 ,比较差异。结果 组织中可以检测到 p53基因 8种改变 ,血浆中检测到 4种 ,与组织相比符合率为 50 .0 %。尿液中检测到 5种符合率 62 .5 %。体液检测突变性质与相应组织是一致的。免疫组织化学染色以阳性细胞占 2 0 %为阳性界值时 ,结果与测序法接近。结论 体液中检测实体肿瘤细胞的突变基因片段用来诊断肿瘤或提供肿瘤生物学行为的相关信息 ,是一种有前途的诊断方法
Objective To investigate the method and significance of detecting DNA of bladder cancer cell in body fluid. Methods The exons 5, 6, 7 and 8 of p53 gene in plasma and urine of 30 patients with bladder cancer were amplified by polymerase chain reaction (PCR). The single strand conformation polymorphism (SSCP) And sequenced the mutation fragments. Comparison of the test results in the control group, the difference. Results Eight kinds of changes of p53 gene were detected in tissues and four were detected in plasma. The coincidence rate was 50.0% compared with that in tissues. Urine detected five coincidence rate of 62.5%. The humoral detection of mutations is consistent with the corresponding tissue. Immunohistochemical staining to positive cells accounted for 20% positive cutoff value, the results and sequencing method close. Conclusion The detection of mutations in solid tumor cells in body fluids is a promising diagnostic method for diagnosing tumors or providing information about the biological behavior of tumors