方法 研究设计 10名志愿者(5男,5女)连续5天服用地红霉素,1天1次,每次500mg(相当于440mg地红霉素代谢物)。与以前的研究(1天2次服用红霉素,1000mg/d;1天2次服用克拉霉素,500mg/d;1天1次服用阿奇霉素,500mg/d,连续3天)相比较。 标本采集 分别在服药后第1,3,5天的0,4,8,24h采集志愿者的血样及尿样,血、尿及多形核细胞溶胞产物于-80℃储存备用。 溶胞产物的制备:PMN从采集的肝素肮凝静脉血中分离。具体步骤见表1。 METHODS: Ten volunteers (5 males and 5 females) were enrolled in the study. Erythromycin was administered for 5 consecutive days in 500 mg doses (equivalent to 440 mg of erythromycin metabolite) once a day. Compared with previous studies (taking erythromycin twice daily for 1 day, 1000 mg / d; taking clarithromycin twice daily for 500 mg / day; azithromycin for 1 day, 500 mg / day for 3 consecutive days). Blood samples, urine samples, blood, urine and polymorphonuclear cell lysate of the volunteers were collected at 0, 4, 8 and 24 hours after taking the drug at -80 ℃ for storage. Preparation of Lysates: PMNs were isolated from collected heparinized venous blood. The specific steps shown in Table 1.