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目的:利用DNA条形码鉴定落葵薯及其近缘植物。方法:对来自不同产地的28份落葵薯及其近缘植物的核基因ITS序列和ITS2序列、叶绿体基因matK序列、psbA-trnH序列和rbcL序列进行PCR扩增并测序,比较不同序列的PCR成功率及测序成功率,对各序列进行种内和种间变异分析,Barcoding gap检验,以及构建NJ树聚类分析,评估不同序列对落葵薯及其近缘植物的鉴别能力。结果:经PCR扩增、测序后发现落葵psbA-trnH序列出现碱基缺失事件,其他序列均扩增、测序成功;matK序列和rbcL序列的测序成功率均为100%,ITS序列和ITS2序列的测序成功率分别为78.75%和64.28%;4条序列中,ITS序列和matK序列的种内和种间距离分别在barcoding gap检验中明显分离;从NJ树来看,ITS序列、matK序列均可区分落葵薯及其近缘植物。结论:建议采用ITS序列及matK序列作为落葵薯及其近缘植物鉴定的DNA条形码。
Objective: To identify the sunflower and its related plants by DNA barcode. Methods: The nuclear DNA ITS sequence, ITS2 sequence, chloroplast matK sequence, psbA-trnH sequence and rbcL sequence of 28 sunflower and its related plants from different habitats were amplified by PCR and sequenced. Success rate and sequencing success rate. The intraspecific and interspecific variation analysis, Barcoding gap test and NJ tree clustering analysis were carried out to evaluate the ability of different sequences to distinguish the sunflower and its related plants. Results: The base deletion event of psbA-trnH was found by PCR amplification and sequencing. The other sequences were amplified and sequenced successfully. The success rates of matK sequence and rbcL sequence were 100%, ITS sequence and ITS2 sequence The success rate of sequencing was 78.75% and 64.28%, respectively. Among the 4 sequences, the ITS and matK sequences were separated within the barcoding gap test, respectively. From the NJ tree, ITS sequence and matK sequence Can distinguish between the sunflower and its related plants. Conclusion: It is recommended to use ITS sequence and matK sequence as the DNA barcodes identified by Fusarium oxysporum and its related plants.