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目的:研究体外培养的小鼠树突状细胞(dentritic cells,DCs)和抗原负载树突状细胞在肿瘤模型鼠体内的分布和形态,为肿瘤的生物学治疗提供形态学基础。方法:分离和培养DC,制备B16黑色素瘤细胞抗原,进行共培养,即为抗原负载的DC,Brdu标记DC和抗原负载的DC,建立B16黑色素瘤小鼠模型,于瘤周围皮下注射Brdu标记的DC和抗原负载的DC。应用光镜、免疫组化方法和透射电镜观察DC和抗原负载DC在肿瘤模型鼠体内的分布和形态。结果:免疫组化染色显示Brdu标记的抗原负载DC与DC比较,体积较大。实验组Brdu标记的DC和抗原负载DC分布的数密度和面密度,分别与对照组比较,有显著差异(P<0.01)。电镜下抗原负载DC细胞与DC比较体积较大,核有切迹,细胞表面的突起较粗大弯曲,形态较成熟。结论:抗原负载DC比DC更易集聚于肿瘤组织周围,推测抗原负载DC比DC可能诱导抗肿瘤效应更强。
OBJECTIVE: To study the distribution and morphology of dendritic cells (DCs) and dendritic cells (DCs) cultured in vitro in tumor models of mice and provide a morphological basis for the biological treatment of tumors. METHODS: DCs were isolated and cultured to prepare B16 melanoma cell antigens for co-culture, which was DC loaded with DCs, Brdu-labeled DCs and DCs loaded with antigen. A mouse model of B16 melanoma was established. Subcutaneous injection of Brdu-labeled DC and antigen-loaded DCs. Light microscopy, immunohistochemistry and transmission electron microscopy were used to observe the distribution and morphology of DC and antigen-loaded DC in tumor model mice. Results: Immunohistochemical staining showed that Brdu-labeled antigen-loaded DCs were larger than DCs. The number densities and areal densities of Brdu-labeled DCs and antigen-loaded DCs in the experimental group were significantly different from those in the control group (P <0.01). Under the electron microscope, the antigen-loaded DCs were larger in volume compared with DCs, and there were notch in the nucleus. The protuberances on the surface of the cells were coarser and more benign. CONCLUSION: Antigen-loaded DCs are more likely to accumulate around tumor tissue than DCs. It is speculated that antigen-loaded DCs may induce stronger anti-tumor effects than DCs.