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目的:观察纳葛胶囊对Wistar大鼠心肌缺血模型中细胞内核转录因子-κB(NF-κB-P65)活性和人核因子κB抑制蛋白α(IκB-α)的RNA表达,从而研究该药对心肌缺血作用的可能机制。方法:健康Wistar大鼠90只,随机分为空白组、模型组、心通口服液阳性对照组及纳葛胶囊高、中、低剂量组。采用高脂饲料联合异丙肾上腺素注射法制备大鼠心肌缺血模型,运用聚合酶链反应技术检测大鼠心肌组织NF-κB-P65及IκB-α的基因表达。结果:纳葛胶囊高、中、低剂量组与各模型组及心通口服液组比较,NF-κB-P65基因表达显著降低,而IκB-α基因表达显著升高,差异均具有统计学意义(p﹤0.05)。结论:纳葛胶囊可以通过降低NF-κB-P65的表达水平,并提高IκB-α的基因表达,调节内皮损伤介导的炎症反应,从而改善具有冠心病心肌缺血的作用。
OBJECTIVE: To investigate the effect of nano-capsule on the expression of intracellular nuclear factor-κB (NF-κB-P65) and the expression of nuclear factor κB inhibitor α (IκB-α) in a rat model of myocardial ischemia in Wistar rats The possible mechanism of myocardial ischemia. Methods: Ninety healthy Wistar rats were randomly divided into blank control group, model group, Xintong oral liquid positive control group, and Na Ge capsule high, medium and low dose groups. The rat model of myocardial ischemia was induced by high-fat diet combined with isoproterenol injection. The gene expression of NF-κB-P65 and IκB-α in myocardium of rats were detected by polymerase chain reaction. Results: Compared with model group and Xintong Oral Liquid group, the expression of NF-κB-P65 gene was significantly decreased and the expression of IκB-α gene was significantly increased in high, medium and low dose Naogic capsule group, the difference was statistically significant (p <0.05). CONCLUSION: Na Ge Capsule can improve myocardial ischemia with coronary heart disease by decreasing the expression of NF-κB-P65, increasing the gene expression of IκB-α, and regulating the inflammatory reaction mediated by endothelial injury.