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【目的】观察益气血补肝肾方对Meis1在围着床期小鼠子宫内膜中表达的影响。【方法】采用免疫组织化学法对Meis1蛋白进行定位及半定量检测;采用免疫印迹法在蛋白水平定量检测Meis1在围着床期小鼠子宫内膜中的表达。【结果】Meis1在围着床期小鼠子宫内膜中呈周期性的表达。免疫组织化学结果显示:妊娠第1天(D1)开始Meis1表达逐渐增强,D4、D5表达显著增强,着床后妊娠D6在蜕膜中的表达增强(均P<0.05)。中药组小鼠子宫内膜中Meis1在D4和D5表达增强,与正常对照组比较差异有显著性意义(P<0.05)。免疫印迹检测结果与免疫组织化学结果相似,中药组小鼠子宫内膜Meis1表达增强,但与正常对照组比较差异无显著性意义(P>0.05)。【结论】益气血补肝肾方改善子宫内膜容受性,促进胚胎着床的作用与其能增强MeisI在子宫内膜的表达有关。
【Objective】 To observe the effect of Yiqi Xue Bugan Shendang on the expression of Meis1 in peri-implantation mouse endometrium. 【Method】 Meis1 protein was localized and semi-quantitatively detected by immunohistochemistry. The expression of Meis1 in peri-implantation mouse endometrium was quantified by Western blotting at protein level. 【Results】 Meis1 was expressed periodically in peri-implantation mouse endometrium. The results of immunohistochemistry showed that the expression of Meis1, D4 and D5 in the first trimester of pregnancy increased gradually, and the expression of D6 increased in the decidua after implantation (all P <0.05). The expression of Meis1 in D4 and D5 in the endometrium of Chinese medicine group increased significantly compared with the normal control group (P <0.05). Immunoblotting results showed that the expression of Meis1 in endometrium of Chinese medicine group was similar to that of immunohistochemistry, but there was no significant difference between the two groups (P> 0.05). 【Conclusion】 Yiqi Xue Bugan decoction can improve endometrial receptivity and promote embryo implantation, which can enhance the expression of MeisI in endometrium.