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应用均匀设计法对影响无柄五加种子萌发的主要因素及水平的作用进行了实验。结果表明,无梗五加种子最佳萌发条件:经改良的液体MS培养基浸泡60h,在—14℃的冰箱中进行层积100d,再将种子在质量分数为3.00mg/L的GA3中浸泡24h后即播种,萌发率达99.7%以上。无梗五加种子需经人工后熟、层积和打破休眠等处理后才能在当年快速萌发。
The uniform design method was used to test the main factors affecting the seed germination and the effects of the level on the seedless. The results showed that the optimal germination condition of Seeds of Adventitious Pestal was as follows: Immersed in modified liquid MS medium for 60h, stratified in refrigerator at -14 ℃ for 100d, and then seeds were soaked in GA3 with mass fraction of 3.00mg / L After 24 hours sowing, germination rate of 99.7% or more. Seagame five plus seeds after artificial ripening, stratification and break dormancy and other treatment in the year after the rapid germination.