近年来报道叶绿酸(CHL,其铜钠盐)在许多短期遗传毒理学试验中具有抗诱变作用, 但还没有在体内抑制致癌物与DNA结合的报道。实验用虹鳟模型,以黄曲霉毒素B_1(AFB_1)和肝DNA的共价结合为终点,检测了CHL的化学预防作用。还用Ames试验检测了CHL对AFB_1和两种杂环胺的抗诱变作用。在体内DNA结合试验中,在1～2 g的鳟鱼 It has been reported in recent years that chlorophyllin (CHL, its copper-sodium salt) has anti-mutagenic activity in many short-term genotoxicity assays but has not been reported to inhibit the association of carcinogens with DNA in vivo. Experimental rainbow trout model to aflatoxin B_1 (AFB_1) and the covalent binding of liver DNA as the end point to detect the chemopreventive effect of CHL. Ames test was also used to test the anti-mutagenic effect of CHL on AFB_1 and two heterocyclic amines. In the in vivo DNA binding assay, 1 to 2 g of trout