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目的:建立基于微流控芯片液-液萃取技术进行人参药材样品前处理的方法。方法:注射泵引入药材初提液及萃取剂于芯片中进行萃取,收集样品溶液进行HPLC检测。对芯片的前处理;药液与萃取剂的流速、流速比;萃取通道的宽度等因素进行考察。结果:纯水清洗后风干的芯片,药液与萃取剂的流速与流速比为v(水饱和正丁醇)∶v(药材初提液)=6(μL/min)∶12(μL/min),萃取通道宽度为200μm的情况下萃取效果更理想。在优化条件下平行试验6次,RSD小于1.2%。芯片萃取所得人参皂苷Rg1、Re、Rb1含量分别为1.05、0.54、1.30 mg/m L,萃取时间仅需2 min,与传统液液萃取相比,芯片萃取的萃取率分别提高了12%、13%、15%。结论:该方法简单、快速、萃取效率高。
Objective: To establish a method for pretreatment of ginseng medicinal samples based on microfluidic chip liquid-liquid extraction. Methods: Syringe pump was used to introduce the crude extract and extractant into the chip for extraction. The sample solution was collected for HPLC. The chip pretreatment; liquid and extractant flow rate, flow rate ratio; extraction channel width and other factors were investigated. Results: The ratio of flow rate and flow rate of liquid and extractant was v (water saturated n-butanol): v (crude drug solution) = 6 (μL / min): 12 ), Extraction channel width of 200μm extraction more satisfactory results. Under optimized conditions, the test was carried out in parallel 6 times, with a RSD of less than 1.2%. Compared with traditional liquid-liquid extraction, the extraction rates of ginsenosides Rg1, Re and Rb1 by chip extraction were 1.05, 0.54 and 1.30 mg / mL, respectively, and the extraction time was only 2 min. %, 15%. Conclusion: The method is simple, rapid and high extraction efficiency.