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[目的]评价比较基因组杂交技术(CGH)在急性白血病细胞遗传学研究方面的作用。[方法]采用常规G显带(CC)和CGH两种方法对30例初治急性白血病患者进行染色体核型研究,荧光原位杂交(FISH)仅用于鉴定。[结果]CC、CGH检测结果一致19例(63.3%),CGH检测异常而CC未检出5例(16.7%),其中CC正常3例,失败2例。CC异常而CGH未检出6例,4例为平衡易位或臂内倒置。CC与CGH均为异常而CGH提供附加信息4例。[结论]在急性髓性白血病和MDS常规细胞遗传学检测中,对因分裂相质量差或分裂指数低而失败的病例、复杂染色体核型及不明来源Mark染色体,CGH是一种有效的弥补方法。联合应用CC、FISH和CGH三种方法系统进行血液肿瘤遗传学检测则更全面而准确。
[Objective] To evaluate the role of comparative genomic hybridization (CGH) in the study of acute leukemia cytogenetics. [Method] Thirty patients with newly diagnosed acute leukemia were studied by conventional G - banding (CC) and CGH methods. Fluorescence in situ hybridization (FISH) was only used for identification. [Results] The results of CC and CGH were consistent in 19 cases (63.3%), CGH was abnormal and CC was not detected in 5 cases (16.7%), of which CC was normal in 3 cases and failed in 2 cases. CC abnormalities and CGH were not detected in 6 cases, 4 cases of balanced translocation or arm inversion. CC and CGH were abnormal and CGH provided additional information in 4 cases. [Conclusion] In acute myeloid leukemia and MDS routine cytogenetic detection, CGH is an effective method to compensate for the cases of complex chromosome karyotypes and Mark chromosomes of unknown origin due to the poor quality of schizophrenia or the low schizophrenia index . Joint application of CC, FISH and CGH three methods of hematological oncology genetic testing is more comprehensive and accurate.