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目的研究纳米氧化锌颗粒对蛋白核小球藻的抑制作用及其机理,为船舶压舱水赤潮藻的杀灭提供理论依据。方法采用小球藻培养计数法和生物测定方法,对纳米氧化锌颗粒抑制蛋白核小球藻的作用及其作用机理进行实验室观察。结果用浓度为0.5 mg/L纳米氧化锌颗粒作用48 h,对蛋白核小球藻的抑制率达到60%。悬液内加入0.5~5 mg/L纳米氧化锌颗粒,作用96 h,蛋白小球藻悬液内抗氧化歧化酶含量升高,叶绿素a和b及总含量均随着投加量的增加而增加。结论纳米氧化锌颗粒对水中小球藻具有一定的抑制作用,其对藻细胞的除藻机理是通过改变蛋白核小球藻叶绿素等生化组成,引起藻细胞抗氧化物酶活性的变化抑制藻类增长。
OBJECTIVE To study the inhibitory effect and mechanism of nano-ZnO particles on Chlorella pyrenoidosa and provide a theoretical basis for the killing of red tide algae in ship’s ballast water. Methods Using chlorella culture counting method and bioassay method, the effect and mechanism of nanometer zinc oxide particle inhibiting Chlorella pyrenoidosa were observed in laboratory. Results With 0.5 mg / L nano-ZnO particles for 48 h, the inhibitory rate against Chlorella pyrenoidosa reached 60%. 0.5 ~ 5 mg / L nano-ZnO particles were added to the suspension for 96 h, the content of antioxidant oxidase in the suspension of Chlorella vulgaris increased, and the total content of chlorophyll a and b increased with the dosage increasing increase. CONCLUSION: The nano-ZnO particles have a certain inhibitory effect on Chlorella vulgaris. The algae-killing mechanism of algae cells is that by changing the biochemical composition of chlorophyll and other chlorophyll, the activity of antioxidant enzymes in algae cells is inhibited and algae growth is inhibited .