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目的探讨建立苯巴比妥、苯妥英钠、卡马西平人血清浓度的测定方法,并应用该方法测定我院患者样本。方法采用高效液相色谱法(HPLC),流动相:乙晴-缓冲盐(30:70);柱温:37℃;检测波长:220nm;流速:0.7mL·min-1。含量测定分析采用内标对比法,杂质检查分析采用归一化法。结果该方法测定苯巴比妥、苯妥英钠、卡马西平的线性范围分别为4.58～75.88mg·L-1,2.25～35.95mg·L-1,1.26～20.08mg·L-1,日内和日间RSD均小于10%;回收率可控制在85%～115%之间。结论该方法具有良好的准确性、精密性和较高的灵敏度,且简便、快速、稳定。
OBJECTIVE To investigate the method for the determination of phenobarbital, phenytoin sodium and carbamazepine in human serum and to determine the sample of patients in our hospital by this method. Methods High performance liquid chromatography (HPLC) was used. The mobile phase was acetonitrile-buffered saline (30:70). The column temperature was 37 ℃. The detection wavelength was 220 nm. The flow rate was 0.7 mL · min-1. Content determination analysis using internal standard contrast method, impurity analysis using normalization method. Results The linear range of phenobarbital, phenytoin sodium and carbamazepine were 4.58 ~ 75.88 mg · L-1, 2.25-35.95 mg · L-1 and 1.26 ~ 20.08 mg · L-1, respectively. RSD less than 10%; recovery rate can be controlled between 85% and 115%. Conclusion The method has good accuracy, precision and high sensitivity, and is simple, rapid and stable.