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诱导型启动子能灵活、精确的调控外源基因的表达,推动了植物基因工程技术的发展.在前期研究中,我们发现了一个萜类合成酶基因LOC_Os02g36140,该基因的表达受茉莉酸甲酯(MeJA)诱导.在本研究中,我们通过PCR技术分离了该基因5'端的2 272 bp启动子序列,命名为PRJA140,构建了PRJA140启动子驱动gusA基因表达的融合载体,转化水稻后对该基因的启动子活性进行分析.MeJA和白叶枯病菌处理转基因水稻后发现,和对照相比,转基因水稻根和叶片中GUS基因的表达明显上调,说明PRJA140启动子能被MeJA和白叶枯病菌诱导,是一个诱导型启动子.“,”Inducible promoter can flexibly and accurately regulate the expression of exogenous genes and promote the development of plant genetic engineering technology.In the previous study,we found a terpene synthase gene LOC_Os02g36140,the expression of which was induced by methyl jasmonate (MeJA).In this study,we isolated the 2 272 bp promoter sequence at the 5'terminal of LOC_Os02g36140 by PCR technology,which was named as PRJA140.In addition,a fusion vector driven by PRJA140 promoter to drive gusA gene expression was constructed,and the promoter activity of this gene was analyzed after rice transformation.After the transgenic rice was treated with MeJA and bacterial blight,we found that the expression of GUS in leaves and roots of the transgenic rice was obviously increased when compared with the control,indicating the PRJA140 promoter could be induced by MeJA and bacterial blight and it might be an inducible promoter.