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目的 :建立一种用HPLC分离测定Legalon胶囊、益肝灵片、复方益肝灵片中水飞蓟宾、异水飞蓟宾、水飞蓟亭、水飞蓟宁含量的方法。方法 :以Shim -PackVP -ODS ( 1 50mm× 4 6mm ,5μm)及预柱 ( 1 0mm× 4 6mm ,5μm)为分析柱。甲醇与混合溶剂水 -二氧六环 ( 9∶1 )按梯度洗脱为流动相 ,流速为 1 5mL·min- 1,检测波长为 2 88nm ,柱温为 4 0℃。结果 :样品中各组分的平均回收率分别为水飞蓟亭 99 66% ,水飞蓟宁 99 4 8% ,水飞蓟宾 1 0 0 0 % ,异水飞蓟宾98 72 %。结论 :该法同时测定 4个主要有效成分的含量 ,便捷、灵敏、准确。
OBJECTIVE: To establish a HPLC method for the determination of the contents of silybin, isosilyin, silymarin and silymarin in Legalon capsule, Yiganling tablet and Fufang Yiganling tablet. Methods: Shim-PackVP-ODS (150mm × 46mm, 5μm) and precolumn (10mm × 46mm, 5μm) were used as the analytical column. Methanol and mixed solvent water - dioxane (9: 1) gradient elution as the mobile phase, the flow rate of 15mL · min-1, the detection wavelength of 2 88nm, the column temperature was 40 ℃. Results: The average recoveries of each component in the sample were 99.6% for silymarin, 99.48% for silymarin, 100% for silybin and 98.72% for silybin. Conclusion: The method of simultaneous determination of the content of the four main active ingredients, convenient, sensitive and accurate.