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目的:利用PCR array技术检测脾虚痰浊动脉粥样硬化巴马小型猪冠脉应激反应与脂质转运和代谢相关基因变化,探讨脾虚痰浊冠状动脉粥样硬化发生的病理机制。方法:随机将10只健康的广西巴马小型猪分为正常组和模型组,每组5只,正常组给予普通饲料饲喂,模型组给予球囊拉伤术联合高脂饮食饲喂进行脾虚痰浊动脉粥样硬化模型复制,实验24周后,观察巴马小型猪行为学变化。呼吸机麻醉后取材,分离血清和冠状动脉,测定血清血脂、hs-CRP、IL-6水平,HE染色观察冠状动脉组织形态学变化,对模型进行评价。应用PCR array技术观察巴马小型猪冠脉应激反应与脂质转运和代谢相关基因变化。结果:造模24周后,巴马小型猪出现食少且不欲饮食、神疲乏力、倦怠等明显脾虚证候,与正常组比较,模型组血清血脂、hs-CRP、IL-6水平显著升高,HE染色结果表明,模型组小型猪血管内膜损伤严重,提示模型复制成功。PCR array结果显示,与正常组比较,与应激反应相关的25个基因中,上调11个,下调3个,与脂质转运和代谢相关的15个基因中,上调5个,下调5个。结论:脾虚痰浊巴马小型猪冠脉应激反应与脂质转运和代谢相关基因变化可能是脾虚痰浊冠状动脉硬化发生的病理机制。
OBJECTIVE: To detect the changes of coronary artery stress response and lipid transport and metabolism related genes in spleen-deficiency phlegm-turbid atherosclerosis parma piglets by PCR array technique and to explore the pathological mechanism of coronary atherosclerosis. Methods: Ten healthy Bama miniature pigs were randomly divided into normal group and model group, with 5 rats in each group. The rats in normal group were fed with common diet. The model group was given balloon injury combined with high fat diet for spleen deficiency Phlegm-turbid atherosclerosis model replication, 24 weeks after the experiment, observe the Bama miniature pig behavior changes. Ventilator was taken out after anesthesia, serum and coronary artery were separated, serum lipids, hs-CRP and IL-6 levels were measured. Morphological changes of coronary artery were observed by HE staining and the model was evaluated. PCR array technology was used to observe the changes of coronary artery stress response and lipid metabolism and lipid metabolism in Bama miniature pigs. Results: After 24 weeks of modeling, the Bama miniature pig showed symptoms of spleen-deficiency syndrome such as eating less and not wanting to eat, fatigue, tiredness and so on. Compared with the normal group, the levels of serum lipids, hs-CRP and IL-6 in the model group were significantly The results of HE staining showed that the intima of piglets in the model group were severely damaged, suggesting that the model was successfully replicated. The results of PCR array showed that among the 25 genes related to stress response, 11 were up-regulated and 3 down-regulated. Among the 15 genes involved in lipid transport and metabolism, 5 were up-regulated and 5 down-regulated. Conclusion: The changes of coronary artery stress response and lipid transport and metabolism related genes in spleen deficiency phlegm Zumaba mini - pigs may be the pathological mechanism of coronary artery atherosclerosis due to spleen deficiency and phlegm.