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植物名称:凤仙花(Impatiens balsamina)。材料类别:顶芽。培养条件:以MS为基本培养基,附加:(1) BA 0.5mg/L(单位下同);(2) BA 0.1+IAA 0.01+GA 0.01。蔗糖3%,琼脂0.6%,pH5.8~6.0。温度25±2℃,照光10~12小时,光照度约1500lx。生长与分化情况:将无菌苗从靠近子叶处切
Plant Name: Impatiens balsamina. Material category: Top buds. Culture conditions: MS as the basic medium, add: (1) BA 0.5mg / L (unit the same below); (2) BA 0.1 + IAA 0.01 + GA 0.01. 3% sucrose, 0.6% agar, pH5.8 ~ 6.0. Temperature 25 ± 2 ℃, light 10 ~ 12 hours, light intensity of about 1500lx. Growth and differentiation: sterile seedlings cut near the cotyledons